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Beyotime
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Beyotime
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Journal: CNS Neuroscience & Therapeutics
Article Title: Zinc Overload in Microvessels Contributes to Blood–Brain Barrier Disruption by Activating the JAK2 Pathway After Cerebral Ischemia/Reperfusion
doi: 10.1002/cns.70885
Figure Lengend Snippet: Conditional knockout of ZnT3 inhibited the phosphorylation of STAT3 in induced mitochondrial apoptosis in cerebral ischemia/reperfusion mice. (A, B) Western blot analysis of p‐STAT3 expression in isolated mitochondria from ZnT3‐cKO or control mice after 60‐min ischemia followed by 24‐h reperfusion ( n = 6 per group). (C) Representative JC‐1 red fluorescence images showing mitochondrial membrane potential in mitochondria isolated from ischemic hemisphere. Scale bar: 100 μm. (D) Quantification of JC‐1 red/green fluorescence ratio in mitochondria isolated from ischemic hemisphere ( n = 3 per group). (E) Quantitative analysis of mitochondrial ROS levels assessed by MitoSOX staining ( n = 3 per group). (F, G) Western blot analysis of Cytochrome C (Cyt C) expression in brain mitochondria from ZnT3‐cKO or control mice with or without ZnCl 2 treatment ( n = 6 per group). Data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus Control + Saline; #### p < 0.0001 versus cKO + Saline.
Article Snippet: Mitochondrial superoxide levels were detected using the
Techniques: Knock-Out, Phospho-proteomics, Western Blot, Expressing, Isolation, Control, Fluorescence, Membrane, Staining, Saline
Journal: International Journal of Pharmaceutics: X
Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect
doi: 10.1016/j.ijpx.2026.100487
Figure Lengend Snippet: Neutralization of merozoites. A Purification of infected erythrocytes using the Percoll separation solution, displaying infected (black arrow) and normal (red arrow) erythrocytes. B Confocal fluorescent images of merozoites (blue), PECm-Allicin@LM (red), and their colocalization (purple). Scale bar = 50 μm. C Representative scatter plots of Hoechst 33342/CFDA-SE for the invasion test of merozoites and normal erythrocytes after drug treatment. D Intrusion rate (the percentage of cells in the Q2 area in each group relative to that in the Q2 area in the Model group). Data are presented as mean ± SEM ( n = 3), * P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: The
Techniques: Neutralization, Purification, Infection